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In order to optimize and speed-up the analysis, tally (Davis et al., 2013) is being used for de-duplicating the sequence fragments.
Thus, the original foreign DNA acquisition event did not rearrange or duplicate the sequence of the target site.
After sequence alignment and removing potential PCR duplicates, the sequencing depth varied from 8.2× (CS) to 12.4× (WR), which was sufficient for CNV detection, and the average coverage with respect to the chicken reference genome sequence was 97.2% (Table 1).
We correlated divergence of WGD duplicates at the sequence and network level.
SAMtools v0.1.7 [ 31] was used to remove potential PCR duplicates among the sequence reads and to call SNPs.
To remove the PCR duplicates of the sequence reads, which can be generated during the library construction process, the rmdup command of SAMtools [ 39] was used.
This can help in reducing the redundancy of sequencing identical viruses by identifying those with unique genetic features and not duplicating the same sequences.
Samples with known genotypes for the described genes, previously tested in duplicate using the sequencing methods, were employed for validation of the assay.
In the case of duplicates, the genomic sequence was given preference.
For the remaining duplicates the shorter sequence of each pair was dropped from the analysis (LarsZAP19, LarsNX24, LarsZAP11).
Typically, unlike other known transposable elements, Helitrons do not contain repeats of their terminal ends and do not duplicate the host sequence upon insertion (Kapitonov and Jurka 2001).
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