For the characters in "Bambi," Marc Davis studied a book of babies' faces to duplicate the expressions in Bambi and Thumper, she said.
All specimens were tested in duplicate, and the expression of SYK and FYN consistently co-varied, with a correlation of 0.28 across SCLC specimens.
Quantitative real-time PCRs were performed with SybrGreen (Applied Biosystems, Darmstadt, Germany) in duplicate and the expression of analyzed genes was normalized to the average expression of the housekeeping gene 18S.
Triplicate samples from each genotype were amplified in duplicate and the expression levels of the genes of interest were normalised to the housekeeping control gene β-actin and analysed as previously described [ 13].
Three independent biological replicates (different from those used for microarrays) were used and all reactions were run in duplicates for all the expression analysis of genes and miRNAs.
This atlas contains custom-designed whole-genome gene expression arrays from mRNAs derived from 79 human and 61 mouse tissues and cell types performed in duplicates and evaluates the expression of 44,775 human and 36,182 mouse transcripts.
To know the functional redundancy/diversification of duplicated ARM genes, the expression profiles were generated for 12 pairs of duplicated genes, covering the spectrum of abiotic stresses as well as vegetative and reproductive developmental stages (Fig. 8).
The average threshold cycle for each gene was determined from duplicate reactions and the expression level was normalized to glyceraldehyde 3-phosphate dehydrogenase (GAPDH).
Considering that the three selected miRNAs shared PTEN as target we performed a tissue microarray analysis of these 64 cases of breast cancer, in duplicate, to evaluate the expression of this protein in both group of patients (metastatic and metastatic breast cancer).
In our case, especially with the evidence that NLRP5 and NLRP14 genes are significantly involved in different developmental stages of embryos, we hypothesize that the functional divergence of oocyte-specific NLRP duplicates might be derived by the expression specialization of duplicate genes, which could provide the mammals reproductive advantages such as in adaptation.
