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Defined tissue aliquots were homogenized in saline/0.04% Tween-80, and 10-fold serial dilutions were plated in duplicates onto Middlebrook 7H10 agar plates containing 10% OADC (Difco).
DNA was extracted from all four fecal samples, the full-length 16S rRNA gene was amplified and further pre-processed as described by Rajilic-Stojanovic and colleagues [23], before being hybridized in duplicates onto HITChip arrays.
Homogenized samples were 10 fold serially diluted in sterile saline up to 108 and serial dilutions (dependent on sample) spotted (50 µl) in duplicates onto CHAB quad plates, with plates allowed to dry without spreading.
Hybridization was performed in duplicates onto Illumina Mouse-6 v1 Expression BeadChips according to manufacturer's instructions.
The clones were arrayed in duplicates onto eight high-density membranes that were screened with seven single-copy gene probes.
The zones of lysis generated by these viral particles were scrapped and deposited in duplicates onto membranes.
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Sample mixtures were boiled at 95°C for 5 min, and then denaturated samples were loaded in duplicate onto 10 well 12% SDS-PAGE.
For each clay mineral, 100 μL of the diluted adsorbent alone and the sample LPS mixture was applied in duplicate onto the 96-well-plate.
An embedded model of the M3 screw seat, part O and I were installed on the XY plane (Fig. 13 a1 and a2)) and duplicated onto the planes.
It was carried out by spot assay in duplicate onto YPD plates containing Kan within various concentrations of ethanol 1, 3, 5, 7, 10 and 15% (v/v), respectively.
In brief, urine samples were mixed with the indicated array-specific binding buffer and loaded randomly in duplicate onto the pre-equilibrated ProteinChip surface, in order to minimize any error source.
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