As a result, 240 duplicated genes were identified on the basis of an expectancy value less than 10-50 as proposed by Friedman and Hughes [ 20].
Onyx was not aware at the time, however, that scientists at Sunway had duplicated the approach on the basis of a paper published by Onyx scientists, according to Sunway executives and to Frank McCormick, Onyx's founder.
We characterized the 3R-WGD-derived duplicate genes on the basis of several properties of the encoded proteins.
Of these, 35 were excluded, (duplicate patients on the basis of matching demographic data), leaving a final sample of 7518 patients.
All measurements, performed in duplicate, were normalized on the basis of protein concentration.
Amplifications by duplication or triplication were defined on the basis of duplicate or triplicate of at least two colour sections observed on the abnormal colour spectrum and pseudocolour profile of a derivative chromosome.
Finally, the PFGE profiles with the additional XbaI bands were easily explained on the basis of the duplicated genome sequence.
Results obtained by above three methods were combined, and duplicate records, which were identified on the basis of locus tags, were removed.
After removing 1383 duplicates and excluding 2439 references on the basis of title and abstract screening 130 full texts were screened, of which 120 full texts were successfully retrieved, as 8 articles had no full text and 2 were irretrievable.
Moreover, to verify whether the information had been recorded accurately, a duplicate collection of data was made on the basis of the clinical histories of 5% of cases selected at random (520 cases overall).
