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Familial segregation analysis of markers inside the duplicated area indicated the presence of two copies of the maternal alleles and one copy of the paternal allele, and FISH analysis confirmed an in situ duplication.
The homology between the 5' region of PKD1 and these six pseudo genes ranges from 94.5 to 96.7% in the duplicated area and almost 100% for the coding sequence [ 8].
In agreement with this result, we note that the duplicated area within both LHC and PSBS sequences extends substantially beyond the shared CB-TM helices and is not homologous between the two groups.
Using this sequence as a guide, we next amplified the region located between the genes encoding for cytochrome b and 12SrRNA (i.e., the entire region spanning the duplicated area) in three overlapping fragments using the primer pairs b3/SlMCR-H171B b3/SlMCR-H171BlMCR-H194A and SlMCR-L160B/SlMCR-H194A one brown and one blue-footed booby.
The observed gene order surrounding the duplicated area was identical to the albatross gene order [ 5] except that only a partial cytochrome b copy was found in boobies, whereas a partial copy and a separate, but degenerate cytochrome b copy was found in albatrosses.
Similar(54)
Regions that do not have significant BLAST hits may still represent the remnants of duplicated areas.
Gm04. 3, Gm06. 1, Gm08.1, Gm11. 1, and Gm18. 1 were found as single copy in their duplicated areas.
Eventually, the duplicated areas shared by all families were narrowed to an ~100 kb interval that contains only one locus, Brachyury [ 68, 69].
Company executives, who declined to discuss specific numbers, acknowledged that they had several duplicate areas of business, as well as buildings and support services, that could be eliminated.
Excluding some redundant BAC clones with the same 3FTx toxin gene restriction fragments, we obtained 25 RFLP-unique 3FTx toxin BAC clones, indicating there are at least 13 and 12 segmental duplicate areas of 3FTx toxin genes, respectively, in B. multicinctus and N. atra.
Then the pod would be duplicated in other areas.
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