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This may be because the contribution of functional compensation by young duplicates cancels the contribution of higher intrinsic importance of ancient duplicate, which is consistent with the duplicability hypothesis [ 9].
Serum creatinine measurements were calibrated at the Cleveland Clinic laboratory-reference laboratory, where serum creatinine levels were measured again using the Roche enzymatic creatinine assay (in duplicate) which is traceable to the National Institute of Standards and Technology creatinine reference measurement [ 11].
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These EST were used to construct cDNA microarrays consisting of 7,741 cDNAs spotted in duplicate, which were used to investigate the regulation of gene expression during fertilization and early seed development events from 0 to 22 days after pollination (DAP).
The components of this matrix M × 1 as shown in Fig. 3 present the number of machine duplicates which is removed from cells and moved to machine depot.
The components of this matrix M × 1 as shown in Fig. 2 present the number of machine duplicates which is returned from machine depot to cells.
11 of the selected genes (22%) are duplicated, which is higher than that observed in the whole genome (17%) (p-value = 0.33 in Fisher's exact test).
Then, α WGD duplicates were updated according to another study [ 44], to exclude tandemly-duplicated WGD duplicates which were shown to have very similar evolutionary patterns with tandem duplicates [ 45].
In two representative angiosperm genomes, Arabidopsis (Arabidopsis thaliana) and rice (Oryza sativa), we identified the ohnologs that were also locally duplicated, which were named locally duplicated ohnologs (the remaining ohnologs were named nonlocally duplicated ohnologs).
Among StNAC genes, 20 were found to be segmentally duplicated, which are located on duplicated segments on chromosomes 2, 3, 4, 6, 7, 8, 10, 11 and 12 (Table 1 and Fig. 2).
The discrepancy may most probably be due to the similarity cutoffs used to determine duplicates, which are reflections on the age of duplications.
PCR duplicates, which were produced during library preparations, were removed using MarkDuplicates from Picard program (https://broadinstitute.github.io/picard/) as well as PickingBases, newly developed by one of us (KK) (see Supplementary Perl Scripts S1).
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