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SPR experiments were performed in duplicate using a Biacore3000 instrument (Biacore, Inc).
All experiments were run in duplicate using two different lots of shrimp.
The VRC was measured, at least in duplicate, using gating strategies as previously described [19].
The rs1024611 and rs1799750 SNPs were genotyped in duplicate using the tetra-arms technique [58].
The assay was performed in duplicate using the Eppendorf RealPlex 4 as previously described [47].
The real time reaction was performed in duplicate using FastStart SYBR Green Master (Roche).
Gene expression was measured in duplicate using 5ng equivalent cDNA per reaction well.
All reactions were performed in duplicate using olive flounder β-actin mRNA as an internal control.
All assays were performed in duplicate using the primer sequences in Table S5.
Test sera were titrated in duplicate using twofold serial dilutions starting at 1∶100.
Amplification reactions were performed in duplicate using 50 ng of each cDNA.
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