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Immunohistochemical analysis was performed on duplicate tissue sections of peri-implant bone from each experimental specimen (9M and IR randomly chosen).
Samples were scored from duplicate tissue culture wells.
Total sugars were obtained by adding 150 mL 0.1 M HCl to duplicate tissue samples and allowing hydrolysis of sugars for 10 min prior to methanol extraction.
Duplicate tissue samples were harvested for Na+ analysis (described above).
Tumor samples were represented in duplicate tissue cores with a diameter of 1 mm.
Both non-infected control and infected MDM were prepared in adjacent duplicate tissue culture plate wells.
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In the first step, NMD is inhibited in duplicate tissue-culture plates.
In the first step, NMD is inhibited in duplicate tissue-culture plates of each analyzed cell line.
Tissue engineering constructs that effectively duplicate natural tissue function must also maintain tissue architectural and organization features, particularly the integration of multiple cell types preserving distinct, integrated phenotypes.
Briefly, duplicate core tissue samples (0.6 mm diameter), were taken from selected areas of maximum cellularity for each tumour with a tissue arrayer instrument (Beecher Instruments, Silver Spring, MD, USA).
All experiments were done at least in duplicate using tissue from different animals.
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