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Some of such copies have been retained in duplicate through evolution [8].
Because of a round of genome duplication occurred at the base of teleosts radiation, several zebrafish genes have been retained in duplicate through evolution.
On the contrary, other genes are prone to duplicate through small-scale events.
To determine the detection limit of the assay, 10-fold serial dilutions of the standard plasmid, (10 to 10 copies/μl in ultrapure water), were run in duplicate through the SYBR Green real-time RT-PCR assay.
Quantification of the measured methylation level was done after these known DNA standards and mixtures were processed in duplicate through all workflow steps starting from pre-amplification to sequencing.
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Genes can be duplicated through tandem duplication and retrotransposition.
The founder of the array of F3'5'Hs on chr6 was initially duplicated through tandem gene duplication.
Genes are duplicated through two main duplication mechanisms; DNA-mediated and RNA-mediated [ 27, 28].
From this initial chromosome organization, the ancestral diploid genome was duplicated through Whole-Genome Duplication (WGD) then fragmented giving rise to an intermediate ancestor with 2n = 2× = 24 chromosomes [ 11].
Trans-NATs expressed antisense of pseudogenes can be duplicated through events of genomic duplication.
The clustering of the OLFR genes in chromosomal regions also suggests this gene family may have been duplicated through in situ tandem duplications [ 13].
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