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All experiments were independently performed in duplicate three times, each time using 1μg of template RNA.
From stage 7 to 10A (approximately 3 hours after stage 6) the follicle cell chromosomes continue to duplicate three times to generate polyploidity (endocycles).
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This analysis indicates that ancestral AGO gene may have required around 2 million years to duplicate four times after divergence from the unicellular green algae (Additional file 5).
All these controls were resuspended in spotting buffer to a final concentration of 50 ng/ml and spotted in duplicate, four times on each microarray slide.
The P. tetraurelia genome has been duplicated three times and retains many duplicated sequences[ 9].
UIDAI will de-duplicate three times over, accuracy that is vital in a country which has had a massive population migration in the past decade and welfare programmes that now total $60bn in value, says Ashok Dalwai, deputy director general of UIDAI.
By performing this feature augmentation, the feature space is duplicated three times.
One of the masks is my face duplicated three times".
It was an opportunity to push the boundary and use a new technology.One of the masks is my face duplicated three times.
This is duplicated three times and panned left, center, and right with differing octaves to add even more power and width during the course of the drop.
The phylogeny presented in Fig. 8 suggests that this gene has been independently duplicated three times.
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