The curves of calibration were obtained injecting each level in duplicate, and the response function was found to be linear with coefficients between 0.998 and 0.999.
We then fit the following model using the generalized linear model with the logit link function in the R programming language: (11) Duplication ∼ log(Degree Centrality) + Duplicated Neighbors + log(Degree Centrality) : Duplicated Neighbors The response was coded as 0 or 1, corresponding to absence or presence of a duplicate paralog from the given WGD, respectively.
Duplicates indicate the response of multiple probes for a given gene.
If there were inconsistencies in duplicate case reports the response was changed to 'Unknown'.
The CL response was monitored at 37°C for 60 min, integrated and expressed in Relative Light Units as the mean of the response from duplicate wells.
Intra-lab differences of the response rates between duplicate runs were in the range of 0% to 6.7% with the width of the 95% CI all being smaller than 30%.
In agreement with our annotation, the induced genes include the earlier-mentioned duplicate copper response system, consisting of a CopZ2 chaperon (SLI0895), a CsoR2 response regulator (SLI0893), a CopA2 ATPase (SLI0896), and their predicted CsoR-like operator sequences (fig. 3).
These independent variables were tested in duplicate at three levels, while dry biomass productivity was used as the response.
The 50%, 75% and 90% inhibitory concentrations (IC50, IC75 and IC90) were calculated with a linear interpolation method, using the mean of the duplicate responses.
Remarkably, both biosynthetic systems are physically linked to a duplicate copper response system, consisting of a CopZ2 chaperon (SLI0895), a CsoR2 response regulator (SLI0893) and a CopA2 ATPase (SLI0896).
