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Data are presented to show that the simulation programs can duplicate the experimental observations of other researchers.
It has been suggested that these negative results could have arisen because of a failure to duplicate the experimental conditions described in the original publication by Lombardi et al [10].
To duplicate the experimental conditions of the sAMS inhibitor study, we constrained the substrate uptake based on the medium used in the experiment [ 53].
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During the scanning, stimuli were displayed via a dual-display stereoscopic video projector (VisuaStimDigital, Resonance Technology Inc). in synchrony with functional acquisition that duplicated the experimental computer screen with 500,000 pixels per 0.25 square inch resolution and a refresh rate of 85 Hz.
In the present comprehensive study, the question of pseudoreplication must be addressed (see also the subsection Statistics): rather than duplicating the entire experimental setup, replicates were confined to the (great number of) samples.
We have also showcased the reproducibility of our experimental setup by providing the reviewers several examples of duplicates of the experimental data submitted for publication (3Bdup, 3Cdup, 3Ddup, 4S1dup and 8Bdup).
All the experiments were done in duplicate to minimize the experimental error.
The presented validation exercise demonstrated that the extended bristle friction model can well duplicate experimental results including typical frictional behaviors such as sliding, sticking and stick-slip.
While a close replication attempts to duplicate experimental methods, a conceptual replication uses a distinctly different study designed to test the same hypothesis [ 17].
Each qRT-PCR analysis was performed in triplicate for each of the duplicate experimental sets of cDNA from the six cell lines.
In addition, in order to confirm that the over-expression of the c-Myc protein was still present after thawing the cell line a western blot was done from duplicate cultures derived from the experimental seeding inoculum and grown to mid-exponential phase to obtain the supernatant for analysis shown in Figure 1b.
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