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Duplicate strains from the same patient showed indistinguishable PFGE profiles (data not shown).
Duplicate strains, isolates originating from existing collections and those of doubtful pathogenicity were also excluded.
Duplicate strains, or strains originating from existing collections, were also not included in the study.
For each genetic background, Exo1 phosphorylation was assessed, in duplicate strains, by western blot.
In both cases, we excluded duplicate strains from the same patient.
For duplicate strains that exceeded the threshold, both RAD-seq and WGS data were retained and strain names were labeled with an "r" and "g," respectively.
Similar(48)
In order to avoid the ambiguity associated with heterozygous genotypes we used sequencing primers that spanned the duplication boundary (duplicated strains only).
A duplicate strain and the corresponding specimen of the C. striatus material is also maintained at the personal herbarium and culture collection of Marc Stadler.
Sequencing was done in biological triplicate for strain panels or biological duplicate for all strains except sake strains, which were done as singlets, with paired growth and strain collection for each replicate.
The resulting dataset was verified by two independent curators, who discarded duplicates, laboratory strains, sequences with missing key metadata, and sequences with quality issues.
Three transformants were assayed in duplicate per strain.
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