So if you need to duplicate or transfer files from one to the other, no middle step, you just plug 'em both in to the USB thing and boom, you're copying.
Reactions were performed in duplicate and run on a Step One machine (Applied Biosystems, Foster City, CA, USA).
62 duplicates are excluded in step one.
This procedure allows one to avoid duplicates when uploading mixtures.
MR: Yeah, they had an incredible guitar sound together that no one to this day can duplicate.
The RNA was divided into twelve 14 μl aliquots for duplicate one-step RT-PCR with the outer primers for fragments F1 to F6 and Superscript III One-Step RT-PCR with Platinum Taq High Fidelity Enzyme Mix (Invitrogen, Carlsbad, California, US).
Real-time quantitative PCR was performed in duplicate for each sample using the ABI Step One Fast Real-Time PCR System (aspreviouslyystems) as previously described (Kleiner et al, 2005).
The search in Medline and Embase, described as step one, yielded, after excluding duplicates, 170 publications.
All samples were prepared in duplicate and one set was agitated to simulate mixing in surface waters.
All qRT-PCR experiments were performed in duplicate (two one-step qRT-PCRs).
No one has stepped in to duplicate Pamela Harriman's or Katharine Graham's elegant soirees, and the Iranian Embassy, which once served free-flowing Champagne and caviar, is long shuttered.
