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Each sample was amplified in triplicate and mRNA estimation was made by comparison to duplicate standard curves and normalisation to β-actin [9].
Duplicate standard curves were included in all the qPCR assays.
Duplicate standard curves for each antibody were made with PC-tubulin (final concentration 150 μg/ml, 3% nonfat dried milk in PBS).
Volumetric data sets were reconstructed into standard anatomic orientations, and all attempts were made to duplicate standard in vivo slice orientation for planar data sets.
Each DNA patient sample was analysed in duplicate, standard dilutions of AdV-5 were run in quadruplicates and negative controls were included in each assay.
Similar(55)
Despite the policy change at the federal level, Reynolds claimed that "the state is allowed to set its own standards for service in the National Guard and is not required to duplicate standards for the rest of the U.S. military".
Each plate contained duplicate standards of purified PCR product of known template concentration over eight orders of magnitude to generate a log template concentration standard curve.
All assays were performed with duplicate standards, a non-cDNA control and a positive cDNA control were extracted from the MCF-7 cell line.
Readings of the duplicate standards and samples were averaged and NG2 concentrations determined by interpolation of a 4 parametric curve fit.
Each plate contained duplicate standards of purified PCR products of known template concentration covering eight orders of magnitude to interpolate relative template concentrations of the samples from the standard curves of log copy number versus threshold cycle.
Each plate contained duplicate standards of purified PCR products of known template concentration covering 7 orders of magnitude to interpolate relative template concentrations of the samples from the standard curves of log copy number versus threshold cycle (Ct).
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