Sentence examples for duplicate splice from inspiring English sources
Multiple sequence alignment of all retrieved sequences was performed using ClustalX [30] to identify and remove duplicate, splice variant, reading frame shift, truncated or otherwise non usable MS4A sequences from the dataset.
Mapping artifacts can create false exon skipping events, due to incorrect or duplicated splice junctions or incorrectly reconstructed exons.
Exons with multiple duplicate functional splice sites (MS and IR exons) less frequently shared all splice sites with the informant species.
Estrogen receptors in Xenopus: Duplicate genes, splice variants, and tissue-specific expression.
Remaining duplicates were splice variants or contained sequence polymorphisms or sequencing errors exceeding a base difference of 1% of the total sequence.
Although the composition of the dynactin complex in vertebrates gradually became apparent, a thorough analysis of the complex and its subunits in terms of gene duplicates, alternatively spliced isoforms, and phylogenetic evolution is still missing.
1.3 ng of cDNA were analyzed in duplicate to quantify spliced and unspliced forms by qRT-PCR.
If the duplicated gene has multiple splice variants with different binding interactions and knockouts of different splice sites between the two duplicates occur, then the result would be subfunctionalization of the duplicates with the ultimate retention of both.
A CD-HIT [ 77] was performed on the proteome set with 99% identity cut-off to obtain a non-redundant dataset that is devoid of splice variants, duplicates and polymorphisms.
Additional studies of expression may reveal subfunctionalization with one duplicate involved in RNA splicing and the other involved in organelle movements, further highlighting the benefit of using a teleost model to study evolutionary consequences of gene duplication.
An analysis of known duplicated and alternatively spliced genes in Drosophila suggests that the use of this method cannot fully exclude misclassification, but that the frequency of false calls should overall be low (see Methods).
