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Duplicate slide staining with primary antibody omitted was routinely performed to provide a negative control.
Independent dilution series were generated and spotted on a duplicate slide.
For each duplicate slide, 25 cells were analysed.
Images of 100 randomly selected cells (50 counts on each duplicate slide) were analyzed for each sample.
Measurements were again repeated on three randomly chosen cells per duplicate slide, thus giving a total of six measurements growth condition.
Concentration matched rabbit IgG (1 μg/ml) was used on a duplicate slide, instead of the primary TACC1 antibody, as a negative control for immunostaining.
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Two researchers independently analyzed duplicate slides in each experiment.
Our service autofaxes all reports and can provide duplicate slides and articles upon request.
Duplicate slides are prepared for each sample (Bajpayee et al. 2005).
The counts were scored blindly and counted in duplicate slides.
Duplicate slides were counted blind, and 300 cells per slide were assessed as either apoptotic or non-apoptotic neutrophils.
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