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"In most cases," she said, "we're eliminating blogs that essentially duplicate section fronts.
A further control was the use, on the same slide as the test section, of a duplicate section to which PCR mix was added without Taq and another section added without primers.
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Duplicate sections of peri-impant bone were obtained from each implant sample to evaluate the percentage of stained areas in order to differentiate markers of collagen I and osteocalcin within both experimental conditions (Fig. 6).
CAMs were cut into 12 sections of approximately 0.1 g wet weight (mean = 0.104 g±0.0009 SEM) allowing for duplicate sections to undergo identical treatment regimes.
Duplicate sections of all tissues were immunohistochemically stained by using a mouse-derived monoclonal antibody (P13C11) specific for type A influenza virus nucleoprotein antigen as the primary antibody (SEPRL, Athens, Georgia, USA).
On the scale of 0 7 for combined extent and intensity, the majority of samples that had duplicate TMA core sections to assess had scores for individual sections separated by at most 1 as shown in Table 5, and the average score for duplicate sections was used for each case for statistical purposes.
In order to investigate the presence of viral antigen, duplicate sections of liver, lung, trachea, air sacs, pancreas, duodenum, jejunum, colon, and caecum from all ducks, as well as all other collected tissues from the two control ducks, were processed for IHC using a commercial anti-influenza A nucleoprotein primary monoclonal antibody [46].
The counts were performed in random duplicate sections.
In each experiment, primary monoclonal antibodies were replaced with PBS in duplicate sections for negative controls.
As a negative control, duplicate sections were stained without exposure to primary antibodies.
Duplicate sections were immunohistochemically analyzed to determine the distribution of influenza virus antigens in individual tissues.
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