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The expected distribution of the duplicate runs showed no obvious change.
The duplicate runs on different gels have given us additional confidence in the alignment procedure.
Data from patient and parents were obtained from at least duplicates run on the same day.
Reproducibility is based on duplicate runs and standards.
For a typical result of the floatation tests, Table 2 shows the data for the duplicate run for T1 (without activation) with the computation.
Reproducibility between duplicate runs was evaluated using a binary comparison map.
The PCRs were normalized by their median and the duplicate runs were averaged.
The average difference between the duplicate runs was −0.02 log10 copies/reaction (95% CI: −0.06 – 0.03).
The results are presented as a mean±SD of two duplicate runs from a representative experiment.
PCR expression values were averaged between duplicate runs, mean-normalized and entered into the Cluster program [41] as log2 values.
Duplicate runs of 10 000 iterations produced almost identical results.
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