About a month ago, Batya Ungar-Sargon, a journalist who was writing about this wine tempest for the VinePair website, asked how I would feel if the great wines of the world, often scarce and expensive, could be analyzed by laboratories and duplicated in quantity.
All samples were analyzed in duplicate and average quantities of the gene transcripts were used for calculation.
All reactions were performed in duplicate, and the quantities of target gene expression were normalized to the corresponding GAPDH expression in test samples and plotted.
Each sample was run in duplicate and the transcript quantity was normalised to the mRNA level of cyclophilin A (human, 4326316E and rat Rn00574762_A1; Applied Biosystems).
This DNA was diluted to 3, 1.5, 0.75, 0.375, 0.188, 0.094, and 0.047 ng per μl and each standard and sample was run in duplicate with the final quantity expressed as the mean of both values.
All reactions were run in duplicates with the mean quantity taken.
Each sample was run in duplicates and the transcript quantity was normalized to the mRNA level of cyclophilin A (Applied Biosystems, Foster City, CA).
Each sample was run in duplicates and the transcript quantity was normalized to the mRNA level of cyclophilin A (4326316E, Applied Biosystems), which expression was tested to not be affected by age.
Each plate also contained a ladder of recombinant mouse PrP 23 230) loaded in known quantities in duplicate or triplicate to establish a standard concentration curve with a four-parameter logistic regression.
The mean quantity of each duplicate calculated by the 7900 sequence detection system software was used for further analysis.
Many of the randomly chosen duplicate samples did not contain appreciable quantities of the analytes.
