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The PtGS2 and PtGS1.2 duplicates contain 13 exons and 12 introns, the PtGS1.3 duplicate presents 12 exons and 11 introns, and the PtGS.1.1 duplicate contains 11 exons and 10 introns.
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A recent duplicate present in A. anableps and not in close relatives is LWS S180β.
Compared to single-copy genes, a family of gene duplicates presents a larger mutational target, and a mutation arising in any gene copy can be spread among copies by non-allelic gene conversion, thereby increasing the efficiency of both positive and purifying selection (Mano and Innan, 2008).
Indeed, the analysis of duplicates presented in figure 2 includes both pairs of genes resulting from single duplications and those stemming from genome duplications.
The final filter removes any duplicates present in the candidate publication pool.
Potential technical duplicates present on the array, which might otherwise skew functional interpretation, were removed.
These results contribute to our understanding of the abundance and type of gene duplicates present in cichlid fish lineages.
However, this procedure did not identify all the duplicates present and the second author carried out a manual check that identified an additional 23 duplicates.
Understanding the different types of duplicates present and how they have evolved in an individual species is key to understanding how they contribute to the species' adaptation.
Out of a total of 113243 sequences submitted to the uniqueness test, 52394 were identified with CS duplicates present due to the preferential retention of those sequences.
However, in a natural, between-species comparisons, the gene duplicates present will include those for which two copies are diverged to varying degrees.
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