All assays were run in sequences that include 10 samples, authentic reference standard mixtures of 80 known compounds, pools of all samples and duplicate preparations of the same sample.
All determinations were made in duplicate preparations.
The method imprecision, calculated as the coefficient of variation for duplicate preparations measurements, was 4%.
All samples were prepared in duplicate, and the method imprecision (calculated as the coefficient of variation for duplicate preparations measurements) was 3.7%.
All samples were analyzed in duplicate and the method imprecision (calculated as the coefficient of variation for duplicate preparations measurements) was 2.5%.
Aliquots of identical total RNA biological duplicate preparations used for Affymetrix GeneChip profiling were used for miRNA profiling using the TaqMan Low-Density Array System (TLDA Life Technologiess).
Results were measured in triplicate (n = 3) and then recorded as volume percentage, V, against particle size interval, W and mean size of interval, d i. Representative size distributions are presented from the duplicate preparations (n = 6).
The percentage of myofibroblasts was assessed by counting at least 1,000 total cells and determining the proportion stained positively for SMA in three fields at 200X in duplicate preparations.
All samples were prepared in duplicate, and the method imprecision (calculated as the coefficient of variation for duplicate preparations) was 9.5%, and for samples with Cd content ranging from the limit of detection (LOD) to 0.10 µg/L, the imprecision was 11%.
The chromatogram is completely dominated by GP-tagged 24S-hydroxycholesterol (27.91 ± 0.73 ng/mg, mean ± SD, two mice were analysed in duplicate preparations, Table 1) which, as a consequence of derivatisation, gives two peaks corresponding to the syn and anti conformers of the GP-derivative.
