DATUM INC., Irvine, Calif., a telecommunications equipment company, said it would cut jobs and combine operations in Beverly, Mass., and San Jose, Calif., to reduce costs and eliminate duplicate positions and services.
Merck intends to eliminate duplicate positions at sales and administrative headquarters and in manufacturing and research facilities.
We also included four controls in duplicate, with each duplicate positioned on different 96-well plates and on different HumanMethylation450K BeadChips: three control samples were DNA extracted from blood and one sample was demethylated DNA (from Zymo).
In summary, performing GBS on the maize inbred line B73 produced a highly skewed coverage of genomic positions, which is only partially accounted for by GC bias and duplicated positions.
A fragment of 290 Kb located at 57.74 Mb-58.03 Mb is duplicated in position 58.34 Mb-58.63 Mb, and a 50 Kb fragment from 58.07 Mb to 58.12 Mb is duplicated and turned over in position 58.73 Mb-58.77 Mb on HSA6 (results not shown).
Briefly, stringent filters were applied to each SNP (call rate, duplicated map position, extreme departure from Hardy-Weinberg equilibrium), resulting in 729 068 informative SNPs.
Each chromosome was scanned by eye for duplicate genes positioned in tandem; where the similarity was not obvious from annotation suspected duplicates were tested by reciprocal BLAST and sequence alignment.
This saves one compression write read step in the common case in which a duplicate marked position-sorted file is needed later in the pipeline, and two such cycles if a duplicate marked read-id grouped file is also needed.
In addition, a 5358 bp segment of the duplicated regions between the 208th and 5565th bases of the duplicated regions, was also duplicated at positions 1,307,425 bp–1,312,781 bp in Schu S4.
However, most of the cuts are likely to be duplicated administrative positions.
