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A duplicate portion of cell suspension of each condition was lyophilized to calculate dry cell weight.
MV, MB and AÅ have made substantial contributions to the original conception and design of the duplicate portion study, acquisition of data and contributed to the manuscript.
These include duplicate portion studies of twenty adults (25 60 years) and thirty-seven pensioners (67 years), lacto-vegetarian diets and vegan diets.
van Dokkum et al. reported the content of carbohydrate constituents in the MB of male Dutch adolescents, while other studies have used the duplicate portion technique to validate food composition databases or dietary assessment methods.
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Collection of duplicate portions is known to influence dietary habits [ 29].
Participants were instructed to save duplicate portions from each meal in separate polypropylene resealable bags.
This indicates a satisfactory agreement between the model and the chemically analyzed duplicate portions.
The analyzed manganese intake from duplicate portions provided a slightly lower average daily manganese intake of 2.4 mg/day.
Duplicate portions of the 10 daily menus on the control diet (PO) were collected for chemical analysis.
Cadmium in the duplicate portions was measured using flame atomic absorption spectrophotometry (detection limit 0.001-0.002 μg cadmium/g) [ 15].
Background dietary intake in the SOY study was derived from duplicate portions of both experimental diets in which energy, fat, protein, ash and dry matter contents were analysed.
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