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All reactions were performed in duplicate per experiment, and β-actin was included in all experiments as an endogenous control.
In experiment 2, in each period in vitro gas and CH4 production were measured (in duplicate per period) for mixed diet samples from the same diet as fed to the donor cows using rumen inocula adapted to the respective diets for an average of 16 d.
In the present study, TLDA cards were configured into four identical 96-gene sets, allowing two samples to be run in duplicate per card.
Cellular supernatant was collected for p24 (Gag) ELISA (Perkin-Elmer, Boston, MA) and samples were run in duplicate per manufacturer's protocol.
The cells were plated in duplicate per experiment.
Measurements were taken in duplicate per standardized protocols [ 17, 18].
Similar(28)
Considering the average number of silent sites (315) and the mutation rate and without modeling the equilibrium frequency of silent sites, the rate at which silent substitution occurs per duplicate pair per generation (λ(t)) can be expressed as λ (t ) = 2 * 315 μ e − 2 * 0.77 μ t.
We found 124 subtrees with C. hamatus lineage-specific duplications, with the number of duplicates per gene ranging from 2 to 8.
It is also worth noting that these charts are duplicated per pilot, meaning that on a transatlantic flight you may have four such chart bags, which must be as much a drag on the cockpit's closet space as it is on fuel consumption.
Experiments were performed in duplicates per treatment.
Three biological replicates were analysed in technical duplicates per treatment.
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