Sample mixtures were boiled at 95°C for 5 min, and then denaturated samples were loaded in duplicate onto 10 well 12% SDS-PAGE.
For each clay mineral, 100 μL of the diluted adsorbent alone and the sample LPS mixture was applied in duplicate onto the 96-well-plate.
It was carried out by spot assay in duplicate onto YPD plates containing Kan within various concentrations of ethanol 1, 3, 5, 7, 10 and 15% (v/v), respectively.
In brief, urine samples were mixed with the indicated array-specific binding buffer and loaded randomly in duplicate onto the pre-equilibrated ProteinChip surface, in order to minimize any error source.
These were spotted in duplicate onto each microarray slide.
Briefly, total brain homogenates from the frontal cortex were diluted in working incubation buffer containing HRP detection antibody then loaded in duplicate onto the antibody coated plate.
The cell-agar mixture was plated in duplicate onto dishes containing a solidified.5 ml layer of 0.5% agar-cell culture medium mix.
Briefly, tissues were homogenized in 4.5 mls of saline buffer and serial dilutions were plated in duplicate onto 7H10 Middlebrook agar plates and incubated in an incubator at 37°C for 21 days for cfu determination.
60 µl of the lipid extracts were loaded (30 µl/lane, duplicate) onto a 20×20 cm silica gel plate (805013, POLYGRAM SIL G, MACHEREY-NAGEL) for cholesterol ester, triglyceride, and free fatty acid separation by TLC.
Briefly, each amplification product was used for an elongation reaction using a dye-labeled Jγ specific-labelled primer for the rearrangement analysis and Cδ labeled primer for the δ chain analysis, then heat denatured, loaded at least in duplicate onto a sequencing analyzer (48-capillary 3730 DNA Analyzer - Applied Biosystems).
The PCR products were purified on a Qiagen 8000 robot by using a Qiaquick 96-well Biorobot kit (Qiagen, Valencia, CA) and spotted in duplicate onto Ultra-GAPS glass slides (Corning Inc., Corning, N. Y). by using an OmniGrid Accent (GeneMachines, Ann Arbor, MI), as described previously [40].
