Sentence examples for duplicate on a from inspiring English sources

Real-time PCR reactions were performed in duplicate on a CFX 96 system (Bio-Rad LaboratheiScriptrcules,CA) with the iScript one step RT PCR Kit (Bio-rad).

But they warned that European regulators had no common tools to deal with a major bank failure, and the multigovernment approach taken in the Fortis bailout would be hard to duplicate on a larger scale.

To this end, we applied the HER2 RollFISH probe validated above (consisting of 48 ODNs) to detect HER2 transcripts in 75 samples represented in duplicate on a single tissue microarray (TMA), including normal breast tissue as well as breast cancers with HER2 IHC score ranging from 0 to 3 + (Supplementary Fig. 9a, b, Supplementary Tables 4 and 5, and Methods).

The samples were run in duplicate on a Lightcycler 480 (Roche Diagnostics, Meylan, France) as follows: 15 min at 95 °C, 45 amplification cycles (15 s at 95 °C, 30 s at 63 °C and 30 s at 72 °C) and 10 s at 40 °C.

The samples were run in duplicate on a Lightcycler 480 (Roche Diagnostics, Meylan, France) as follows: for bacterial amoA 15 min at 95 °C, 45 amplification cycles (30 s at 95 °C, 45 s at 54 °C, 45 s at 72 °C and 15 s at 80 °C) and 30 s at 40 °C; for archaeal amoA, 15 min at 95 °C, 50 amplification cycles (45 s at 94 °C, 45 s at 55 °C and 45 s at 72 °C) and 10 s at 40 °C.

In a process similar to printing a photograph, engineers beam high-powered light through a mask-like image of the chip, creating a duplicate on a silicon wafer.

Quantitative PCR were performed using the SYBR GREEN Jump Start kit (Sigma Aldrich) in duplicate on an ABI apparatus using standard procedure.

Real-time PCR was performed in duplicate on an iCyler real-time PCR instrument (Bio Rad Laboratories Inc ,Dr. Hercules, CA, USA) using 2× Universal PCR Master Mix, no AmpErase UNG (AB, Foster City, CA, USA).

Plasma samples were assayed in duplicate on an Advia 2400 Chemistry System (Siemens Healthcare Diagnostics, Deerfield, IL, USA).

Serum glucose was measured in duplicate on an autoanalyzer using the hexokinase method.

All TaqMan assays were run in duplicate on an ABI Prism 7900, applying 40 PCR cycles.