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TLC of each essential oil was performed on silica coated TLC plates in duplicate manner.
All PCR reactions were performed in an independent blinded duplicate manner and for each polymorphism some samples were confirmed by sequencing the PCR products (Abi Prism 310, Applied Biosystems).
RNA samples were independently analyzed by qRT-PCR three times in duplicate manner (column, mean; bars, S.E .. Correlations were obtained using Spearman's correlation.
After the removal of media, the cultures were overlaid with 0.1 ml of each serial 10-fold-diluted virus suspensions in duplicate manner, and incubated for 1 h with rocking at 15-min intervals.
Assay consistency was developed by the use of commercially manufactured control sera of high, medium, and low concentrations in each run and all assays were carried out in a duplicate manner.
MMS2 (UEV1) gene family has four members and all of them were duplicated in pair-wise manner to each other.
I wanted the manner to be good.
No manners to learn.
Use manners to overwhelm their rudeness.
Always use manners to your advantage.
Mycoplasmas duplicate the TO prior to cytokinesis in a coordinated manner with the cell division process [18], [19].
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