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Graticules include 180-degree east and west meridians (consider deleting one of these to avoid duplicate lines).
Do not forget to run the 'overkill' command in AutoCAD to guarantee that any duplicate lines are eliminated.
In total, 12 flats contained 142 lines (128 plus the 14 duplicate lines).
The phenotypes for identical lines (including the five duplicate lines and the two identical genotype lines) were merged together.
After removing duplicate lines and lines with large amounts of missing data, the final data set consisted of 2198 mapped SNPs scored in1807 lines.
One plant from each line of the RIL set including the parents and the duplicate lines were genotyped at 65 single nucleotide polymorphism markers by Sequenom (San Diego, CA).
Similar(51)
Create a duplicate line like the second stroke right underneath it, from left to right.
The company said it will meet those production targets by improving the use of its existing lines and making selective improvements to address bottlenecks rather than creating entirely new duplicated lines.
Analysis of the PCA obtained from the seed samples showed clear differences between both duplicated lines and their respective donors.
Both duplicated lines were significantly affected by genome doubling and, as a consequence, the meiotic behavior changed, and the morphological traits of fruits, seeds and embryos were all altered, as were the metabolic pathways.
Both duplicated lines exhibited a significant reduction in betanin and phyllocactin, with the decrease being more significant in the autohexaploid than the autotetraploid fruit pulp samples (a 1.4- and 7.5-fold decrease in betanin, and a 1.3- and 2.5-fold decrease in phyllocactin, respectively; Figure 5).
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