In cases of duplicated genes of interest such as HMA4 (tandemly duplicated three times in A. halleri), we used only one copy for diversity analysis due to non-unique alignments of Illumina reads and very high sequence identity (99%) in the A. halleri reference genome.
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Large organizations cannot hope to duplicate this lure without creating interest among those who are not offered such rewards.
Each dilution of the standard curves was amplified in duplicate and each sample of interest was amplified in triplicate.
Samples were run in duplicate for each gene of interest.
Each sample was assessed at least in duplicate for each gene of interest.
Samples were measured in duplicate for each gene of interest and the reference gene H2AFZ [ 84] was measured in all samples as normalizer.
The following studies were excluded: (1) case-control study with benign breast disease selected as controls, (2) case-control study with sample size less than 100 in each arm, (3) study with incomplete data of interest, and (4) duplicate publications.
Its work in total will likely be less than the sum of individual efforts because members will duplicate work, pursue their own interests, or even labor at counter-purposes.
Important aspects of the data processing by selecting only the bioactivity type of interest, dealing with duplicates, removing missing data and salt groups, descriptors calculation, and data normalization are handled in a very flexible and consistent manner.
Among such clusters, the homolog showing the highest scoring alignment was labeled non-locally duplicated towards the gene of interest.
