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It is worth noting that duplicate individuals are deleted in the preservation of optimal individuals.
All duplicate individuals were called identically.
Data were further examined and cleaned to identify duplicate individuals, exclusion drugs and data entry error.
Identity by decent was performed to identify and remove duplicate individuals.
We investigated possible overlap of carriers between studies by comparing the year of birth, exact mutation description and the reported ages, to identify potential duplicate individuals.
Although a few polymorphisms were detected in the nrITS sequences of our samples (even in some duplicate individuals of the same species) via direct sequencing, no pseudogenes became evident [ 82].
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Duplicate individual cultures (150 μl volume) were stimulated with one of six different TLR agonists (at the concentrations shown in Table 1) or left unstimulated as negative controls.
After the removal of duplicate individual genotypes and identifying loci that met quality criteria (see Materials and Methods), sufficient data were obtained for 102 biallelic loci in 147 individuals (Arica, n = 37; Las Cruces, n = 95; Niebla, n = 15).
We here provide evidence that the expression of SAM organizers are also altered in ccs52a2-3, anduplicatete individual CLV3-WUS regulatory centers are sometimes generated in the mutant SAM.
The DNA extracts (20 μL/assay) were added to the freeze-dried reagent tubes along with 20 μL of reconstitution buffer and immediately tested in duplicate (individual reaction volume 20 μL; DNA template volume 10 μL/reaction) on the JBAIDS instrument.
Based on 17 duplicated individuals for the 118 SNPs that worked, we estimated a genotyping error of <0.0005%.
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