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Most look-alikes, after being introduced to their duplicate, indicated low to modest levels of anticipated friendship and feelings of familiarity upon meeting, with decreases over time.
Linear regression analysis of the data from two independent experiments carried out in duplicate indicated that the single nucleotide change had no significant affect on promoter activity (p = 0.8).
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However, the high concordance rates among the subset of samples that were genotyped in duplicate indicate that genotyping was generally consistent for other SNPs in the study.
The long branch leading to this second duplicate indicates that it is evolving more quickly than the copy conserved in all Microsporidia, suggesting functional divergence between the paralogs.
The representative calibration curve, obtained by analyzing mixtures of unmethylated and fully methylated control DNA on 4 days in duplicate, indicates the high inter-day repeatability of the method (Fig. 1b).
In contrast to such diversifying processes, the phylogenetic relationships of tandem duplicates from closely related Trypanosoma spp., and conflicting phylogenetic signals from alignments of tandem duplicates, indicated that both concerted evolution and gene conversion affect tandem duplicates in T. brucei.
Surveys of the functional distribution of gene duplicates indicated that pathogens show enrichment for gene duplicates associated with receptor and hydrolase activities, while euascomycete pathogens appeared to have not only these differences, but also significantly more duplicates associated with regulatory and carbohydrate binding functions.
Furthermore, a 257 nucleotide spacer region corresponding to wasp sequences between the two viral segments is also duplicated indicating that the duplicated region was a chromosomal region including the segment, not the segment alone.
We did not detect a GAPF-positive signal in the genomic area known to be duplicated, indicating that the duplication is unlikely to be an inverted repeat (data not shown), but presumably direct.
Abundant variation among gene duplicates indicates that tandem duplication has greater consequence than simply increasing gene dosage; the systematic account given here provides a basis for uncovering the hidden functionality within tandem gene arrays in trypanosomatids.
Both centrosomes duplicated, indicating that the block is not controlled by centrosome:nucleus ratio.
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