Gene duplicates were unified according to the gene name annotation as the mean values of the duplicate expression levels.
Duplicate expression profiles were obtained with the GeneChip Rat 230 2.0 Array from the Affymetrix Co as has been described [ 21].
For all targets with a difference in the duplicate expression of greater than one c(t), a re-analysis in triplicates was performed.
A greater percentage of type I gene and EST-based markers were analyzed in rainbow trout, however, compared to Atlantic salmon which may indicate that duplicate expression is retained to a greater degree within coding vs. anonymous DNA.
Subsequently, the experimental time points were divided into 10 subcategories (5 pairs of each time point's mastic oil treatment and its corresponding control), each consisting of duplicate expression values for each gene.
To evaluate the RT-qPCR data, the mean value of the duplicate expression values (Ct values) was calculated and normalized with the mean Ct value of the reference gene GAPDH.
Moreover, the aforementioned virulence associated genes were identified to be repressed by H-NS [ 13], whereas their duplicates' expression was not significantly affected by H-NS.
For the characters in "Bambi," Marc Davis studied a book of babies' faces to duplicate the expressions in Bambi and Thumper, she said.
The protocol starting from the lysis of all the 48 mycelial samples to analysis of expression of 18 genes in duplicate (1728 expression levels) was carried out in 7 h with 1 to 2 h hands-on time.
Five of the 7 pairs of chromosomal arm regions expressing the highest level of duplicate gene expression in rainbow trout share homologous synteny to the 5 pairs of homeologs with the greatest duplicate gene expression in Atlantic salmon.
