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Duplicate crossing points per marker were averaged per independent experiment for both primer pairs (n=16, including two cDNA dilutions).
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For those who must see a poinsettia for the holiday, they are at the garden's train show, where miniature trains toot along 1,000 feet of track, crossing a duplicate of the Brooklyn Bridge, and chugging by favorite landmarks, like Radio City Music Hall or the Belvedere Castle in Central Park.
A sample was considered positive if duplicate reactions showed amplification crossing the threshold at cycle (CT) of 37 or earlier.
The differences in gene expression were determined by comparing the crossing points of three samples measured in duplicate.
Differences in gene expression were determined by comparing the crossing points of two samples measured in duplicate.
They swing from ropes, fire a small cannon and belt out tunes from a stage set duplicate of a wooden pirate ship with Mr. Davis standing on the bow like Washington crossing the Delaware.
The lineup for that film program also features "The Ballad of Genesis and Lady Jaye," directed by Marie Losier, which documents the love story between Genesis P-Orridge and Lady Jaye Breyer, whose physical features Genesis P-Orridge duplicated through cross-dressing and plastic surgery.
One explanation for the difference might be that the BACs used in both studies were from rainbow trout, so they might be more likely to cross-hybridize with duplicate loci in the homologous species.
Each cDNA sample was tested in duplicate, and the crossing point (Cp) value obtained allowed us to determine the amount of the starting message using the specific standard curve.
The traces identified by these searches were downloaded in.fasta format and cross checked to eliminate duplicate results.
Taryn A. Merkl, an assistant United States attorney, protested to Judge Garaufis that the switch in defense lawyers was tantamount to taking a "second bite of the apple," an effort to duplicate the earlier cross-examination.
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