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Samples of beads of 0.25 0.3 g were placed into glass vials in duplicate containing 3 mL of the solvent mixture (hexane:ethanol:acetone containing 0.63 g/kg BHT, 50 25:250 25
Additional file 17: A figure of the nucleotide alignment of genomic sequence of duplicate containing H4 protein sequences FGRRES_20411 (FGSG_04289) and FGRRES_05491_M.
The first data set consisted of the hybridisation results of the MRSA252 specific spots (75% of the spots in duplicate) containing a reference signal (further indicated as MRSA252 spots).
All reactions were set up in duplicate containing: 1 μl cDNA template (50 ng), 7.5 μl AbsoluteTM QPCR SYBR Green Mix and 1 μl of each primer (3.75 mM) in a final volume of 15 μl.
Real-time reactions were performed in duplicate containing cDNA generated from 10 ng of original RNA template, 300 nM each of specific forward and reverse primers, and 5 μl of iQ™ SYBR Green Supermix (Bio-Rad) in a 10 μl final volume.
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The PtGS2 and PtGS1.2 duplicates contain 13 exons and 12 introns, the PtGS1.3 duplicate presents 12 exons and 11 introns, and the PtGS.1.1 duplicate contains 11 exons and 10 introns.
Each duplicate contains 27 genes, the majority encoding products involved in the biosynthesis of constituents of the LPS.
Regulatory modules alternatively maintained in the promoter of either F3'5'H duplicate contain binding sites for Myb-type transcription factors, drought-inducible cis-elements, and motifs responsive to ABA, methyl-jasmonate, light, and heat stress.
Aliquots of 100 µL were dispensed into Petri dishes, in duplicates, containing brain-heart infusion agar (Difco Laboratories, Detroit, MI, USA) supplemented with 4% (v/v) of fetal bovine serum.
Each of the duplicates contained material pooled from multiple mice.
Confirmation of tandem gene arrays is possible because most tandem duplicates contain sequence variation, often arranged as distinct sequence types.
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