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qPCR reactions were performed in duplicate, consisting of 1 µl DNA template (0.5 ug total), 2 µl each of forward and reverse PCR primers (final concentration of each primer: 100 nM), 10 µl 2X Brilliant SYBR Green qPCR Master Mix (Stratagene, La Jolla CA), 0.3 µl diluted reference dye (Stratagene, La Jolla CA), and 4.7 µl nuclease-free PCR-grade water.
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Each biological duplicate consisted of 9 technical replicates.
All genotype assays contained negative and positive controls, and 10% blinded duplicates consisting of patient samples.
Duplicates consists of records retrieved in two or more databases.
The approach for identifying segmental duplicates consists of identifying pairs of sufficiently similar genes in a phylogeny that fall sufficiently close to their respective corresponding regions in a synteny block.
Pairs of duplicate samples consisting of basal and cambium tissue from the same tree had the highest average genetic distance, while foliage-epicormic pairs had the lowest.
The assay was done in two biological duplicates each consisting at least two technical duplicates.
We analyzed release 5 of the rice japonica genome obtained from TIGR and identified 24 distinct duplicated segments consisting of 4492 duplicated genes covering 261 Mb (70%) of the rice genome.
A duplicate contest consists of a comparison with at least one other table.
Duplicate bridge consists of two or more tables, played for masterpoints and prestige with partners and teams self-selected in advance.
Duplicate reactors, each consisting of two modules, were operated with influent sucrose organic loading rates (OLRs) between 0.04 and 0.42 g COD/l/d.
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