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All assays were performed in duplicate (coefficient of variation = 0.01).
All assays were performed in duplicate (coefficient of variation = 0.01).
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Each sample was tested in duplicate for each gene, and the analysis was repeated for those samples with large variation in their duplicates (coefficient of variation >5%).
Plasma chitotriosidase activity was measured in duplicate and coefficient of variation was less than 5% in all measurements.
Measurements were done in duplicate; the coefficient of variation and day-to-day variation were 6% and 5%, respectively [ 5].
In this experiment, phosphorylation of a human protein by CaMKII was considered significant if the human protein Z-Score was equal or greater than 3, the duplicate spots coefficient of variation less than 0.5, and the background-corrected signal in a CaMKII assay was at least 150% of the background-corrected signal in the no kinase control assay.
All samples were run in duplicate with a coefficient of variation between duplicates of <1.0 cycle.
To ascertain analytic quality all standards, controls and samples were analyzed in duplicate and all duplicates with a coefficient of variation >10% were reanalyzed.
Approximately 30% of overall variation was explained by variation in the molecular behavior of different tissue specimens of the same breast (note the assays were performed in duplicate, and the coefficient of variation (CV) of the assay was small compared to each of these components of variation and so can be ignored).
They were measured in duplicate with a coefficient of variation of 3.9% and 1.9%, respectively.
Each blood sample was measured in duplicate with a coefficient of variation of <5%.
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