Sentence examples for duplicate coat from inspiring English sources

The billionaire's autobiography – which was the happy recipient of a five-star review in the Daily Express this week – is a masterclass in how to lie your way to the top, beginning with his job in a nightclub cloakroom at the age of just 13, where he devised a scam to duplicate coat tickets and pocket the money.

This greater diversity in Rab evolution may reflect the fact that it is difficult to duplicate a coat, a tethering complex, or a SNARE tetramer, as they are encoded by multiple genes.

Duplicate wells were coated with aggregated native IgG1 as a positive control and BSA was used as a negative control.

Estradiol, free estriol, testosterone, progesterone, and cortisol were assayed in duplicate using the Coat-A-Count® radioimmunoassay (Diagnostic Products Corporation, Los Angeles, CA) according to the manufacturer's instructions.

100 μl of diluted blood was added into 96-well round bottom plates (Nunc, Roskilde, Denmark) in duplicate wells pre-coated with the specific antigen diluted in 100 μl medium.

Briefly, 100 μl of samples or standards (0 to 250 pg/ml) was pipetted in duplicate into wells coated with antibody to relaxin and was incubated at 4°C for 12 hours.

Follicle-stimulating hormone and LH: all samples were assayed in duplicate using a commercial Coat a count solid phase Immunoradiometric assay (DPC, Gwynedd, UK).

RA, OA and other disease SFs, and Id1 as a standard were coated in duplicate for one hour.

Serum samples were incubated in duplicate on microtiter plates coated with a specific monoclonal antibody for 2 hours at room temperature.

Concentration of anti-α-gal antibodies in human plasma was analyzed using a similar assay and a standard curve to transform absorbance into immunoglobulin concentration was obtained by coating 8 duplicate wells in every plate with purified human IgG and IgM (500 to 1.5 ng/ml) and performing the protocol described above in the absence of diluted serum.

Substrate surface defects like holes and protuberances were not duplicated during the coating process.