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Duplicate cells were scraped, resuspended and cell viability measured as before.
Individual donors were studied in duplicate; cells from different donors were not pooled in any experiment.
Duplicate cells dosed with 0.00033% H2O2 for 24 h were harvested and lysed as for western blotting.
For the cell viability assay, the cells were examined by counting (in duplicate) cells that excluded trypan blue dye.
Similar(56)
Duplicate cell counts were averaged for 3 experiments.
For each treatment, RNA was isolated from two duplicate cell culture wells after 24 hours of exposure.
For the viral plaque assay, serial tenfold dilutions of each specimen were prepared in HBSS with 2% fetal bovine serum added (HBSS-FBS), and 50 µl of each dilution was adsorbed on duplicate cell monolayers for 1 hour at 37°C.
For known duplicate cell lines, only one of each duplicate was counted for association studies.
Duplicate cell counts were determined daily for 7 days using a cell counter (Coulter, FL, USA).
Fraction of active cells is plotted as mean for two duplicate cell chambers for each condition.
There is good agreement for the majority of pairs of duplicate cell lines.
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