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Fifteen percent of the samples assayed were of known genotype, determined by sequencing, which were unknown to Geneservice, but known at source and 10% were genotyped in duplicate as a quality assurance measure.
A total of 73 20-mer peptides covering SKIP residues 49 to 140 with a one amino acid offset were synthesized in duplicate as a spot array on cellulose membranes (kind gift of Dr. C. Freund, FMP, Berlin, Germany).
Each hybridization was performed in duplicate as a dye swap hybridization.
Each experiment was performed in duplicate as a dye swap to eliminate any labelling bias.
Similar(56)
The HapMap samples were hybridized against each other in duplicate, as was a pool vs. pool hybridization.
Because many of the knocked-out genes are represented in duplicate as both A and a mating type strains, the actual number of knocked out genes screened was approximately 7800.
However, with increasing evolutionary age, we observed a significant increase in the frequency of both partial and chimeric duplicates as well as a concomitant attenuation of duplication spans.
We observed that divergently oriented paralogous gene pairs tend to duplicate as an entity and this duplication is still ongoing after speciation.
A total of 73 overlapping 20-mer peptides covering amino acids 49 to 140 of SKIP with a one amino acid offset were synthesized and assembled in duplicate as an array of spots on cellulose membranes (kind gift of Dr. C. Freund, FMP, Berlin, Germany).
Two of the entries were included in duplicate as an internal control.
Each protein or control was printed in duplicate as an adjacent pair of features.
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