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A total measurement cycle took 11 min, permitting duplicate analysis of 3 samples per hour.
Method imprecision (between-run coefficients of variation [CVs] from duplicate analysis of three quality control [QC] urine pools across 20 runs) was 5.6 12% for ESI, as opposed to 5.3 30% for APCI.
Analytical reproducibility determined by duplicate analysis of 12 random samples revealed good assay consistency for all three analysed antigens, with day to day variation in normal subjects being relatively minor in significance.
Duplicate analysis of 12 mouse urine samples showed good reproducibility (CV 6% within assays).
Furthermore, method performance was carefully monitored using multiple internal standards (5 to 10 depending on method, including analogues, 2H and 13C labeled metabolites) and duplicate analysis of samples.
Duplicate analysis of sample extracts for total As permitted an evaluation of instrumental precision.
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For the BC sample set, Spearman's rank correlation coefficient was calculated on peak intensities in each duplicate analysis for successive subsets of peaks including the 10%, 20 %, 30 to 100%to100%aks with lofest intensity, for all three data sets.
Through automatic duplicate analysis, 50 of the initial 241 articles identified were excluded.
Values are expressed as means of duplicate analysis ± SD.
Average sample spike recovery for As (n = 2) was 112% and relative percent difference of duplicate analysis (n = 2) was 13%.
In addition, much lignin is known to be solubilized under pretreatment conditions [ 25] and it is possible that such solubilization involves the loss or relocation of autofluorescing components.> -wrap-foot> Vareexpressedpressed as means of duplicate analysis ± SD.
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