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Each sample was arrayed in duplicate (also for the TMAs engineered for the extended analyses, see below).
The other duplicate of NADP-ME showed a constant expression level along the developmental gradient, but for the four other enzymes, the second duplicate also showed an increase of expression along the developmental gradient.
Primary chondrocytes from 6 and 12 week rats (n = 6 12 animals per age, 2 experiments in duplicate) also lacked D2 or D3 activity and chondrocytes from 3 week rats had no detectable D2 activity.
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His fellow officers at the Mint presented him with a gold medal, with silver and bronze duplicates also struck.
Surprisingly, the older segmental duplicates also showed evidence of elevated intraspecific gene expression variation albeit typically lower than for the tandem duplicates.
Besides block duplicates, also the number of tandem duplicates is large in the Phytophthora genomes.
The sampling bias of WGD duplicates also may confound our analysis.
First, we identified those duplicated also in at least one of the model fish species.
The number of PCR duplicates also appeared to be affected by individual runs; a consistently fewer duplicates were observed in the second replicate.
Interestingly, these gene duplicates also show testis-specific expression that the authors suggest is evidence of SC resolution (Gallach et al. 2010).
After sorting the mapped reads by the coordinates of the sequence with Picard tools, we labeled the PCR duplicates also with Picard tools (http://sourceforge.net/projects/picard/).net/projects/picard/
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