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Once centrioles have duplicated in S phase, they cannot duplicate again until the next S phase.
If discrepant RLU values were observed in the two tests, samples were tested in duplicate again (maximum three thawing cycles).
For an old centriole to duplicate again, therefore, disengagement of the daughter centriole appears to be the crucial licensing event that allows reduplication [ 4, 5, 31], because immobile Asl incorporation has already occurred.
We therefore propose a "dual-licensing" model of centriole duplication, in which Asl incorporation provides a permanent primary license to allow new centrioles to duplicate for the first time, while centriole disengagement provides a reduplication license to allow mother centrioles to duplicate again.
Taken together, our findings suggest a dual-licensing model in which the recruitment of the immobile fraction of Asl by DSas-4 provides an irreversible primary license to allow newly formed centrioles to duplicate for the first time, while centriole disengagement provides a reduplication license [ 5] to allow older centrioles to duplicate again.
That extra centrioles appeared to be daughters is consistent with the report that, with the exception of certain cell types such as CHO and U2OS, daughter centrioles when separate from their mothers do not mature and duplicate again when cells are in S phase (Lončarek et al., 2010).
Similar(53)
That will never be duplicated again".
Many have two nuclei, instead of the usual one, and within these double nuclei the DNA may be duplicated again.
"These kinds of friendships can never be duplicated again," said Charlie Vitchers, general superintendent of Bovis Lend Lease, the construction company that has supervised the debris removal effort.
The debut went remarkably well, with barely a glitch and with all the accompanying excitement of an opening night that will never be duplicated again.
The β chain gene became duplicated again about 200 million years ago, giving rise to the γ hemoglobin chain, a normal component of fetal hemoglobin (hemoblobin F).
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