Regarding subcellular networks, on the other hand, the genes involved in transcription regulations and signal transduction pathways were found to be over-retained in duplicate after whole genome duplication (WGD) in higher eukaryotes [ 13, 14].
The large subunit underwent a larger number of duplications than the small subunit, which only began to duplicate after the divergence of monocots and eudicots.
The extraction with each reagent was carried out in duplicate after 16 h of end-to-end shaking.
Successive quantitation of drugs from patient urine samples was made in duplicate after 3, 8, 15, and 30 days of storage at room temperature and at 4°C.
LAM antigen concentration in the pleural fluid was measured in duplicate, after a heating step to dissociate antigen and antibody, according to the manufacturer's instructions (Clearview® TB ELISA, ME, USA; see http://www.clearview.com/tb_elisa.aspx).
Because non-sterol regulation of HMGCR has traditionally been studied in fibroblasts [19], we incubated early passage normal human diploid fibroblasts (IMR-90) for 24 hours with 2.0 µM simvastatin + 10% LPDS in duplicate, after which LDL-C (50 µg/ml) was added to the media.
