Sentence examples for duplex unwinding from inspiring English sources
Our data reveal that eIF4H is much less efficient at stimulating eIF4A unwinding activity than eIF4B, implying that eIF4H is not able to completely substitute for eIF4B in duplex unwinding.
In addition, during transcription, negative superstress is generated behind the Pol II enzyme [20] which should temporarily impede the re-association of the two DNA strands and would thus slow down mRNA/DNA duplex unwinding.
(B ) Mechanism of duplex unwinding.
First, Rep helicase shuttling was detected during translocation on ssDNA, not duplex unwinding.
We next examined how the stability of the ternary closed-state complex with ssRNA and the same NTP analogues correlates with the efficiency of duplex unwinding.
Samples were analyzed in a non-denaturing 6% polyacrylamide gel run at 4°C for 60 min. The fluorescence signal of the bound duplex substrate was quantified by using a Typhoon imager (GE Healthcare, UK) to measure the formation of a closed-state complex containing a single-stranded nucleic acid region, indicating duplex unwinding.
Finally, we propose the FeS cluster-containing domain also provides a wedge-like feature that is the point of duplex separation during unwinding.
Replication initiation is a crucial step in genome duplication and homohexameric DnaB helicase plays a central role in the replication initiation process by unwinding the duplex DNA and interacting with several other proteins during the process of replication.
DOI: http://dx.doi.org/10.7554/eLife.06562.002 Replicative helicases and DNA polymerases (DNAPs) are not efficient at unwinding the duplex DNA when they are working independently.
Activators can accelerate initiation by stabilizing the initial RNAP/promoter complex, by stimulating the isomerization of the initial RNAP/promoter complex to RPo (i.e., unwinding the duplex DNA to form the transcription bubble), or both (Li et al., 1997; Roy et al., 1998).
Instead of leaving after G4 unwinding, allowing it to refold, or going beyond to unwind duplex DNA, Pif1 repeatedly unwinds G4 DNA, keeping it unfolded.
