Sentence examples for duplex concentration from inspiring English sources
c. Duplex concentration: 2 μM.
The duplex concentration was 0.3 mM.
The IHF binding site size (n = 8) and duplex concentration ([DNA]) were held as global constants.
The duplex concentration was 1.5 mM, and the samples were suspended in 20 mM sodium phosphate, 100 mM NaCl, and 50 µM Na2EDTA (pH 7.0).
The modified and unmodified samples were dissolved to a duplex concentration of 0.25 mM in 180 μL of 200 mM NaCl, 50 μM Na2EDTA, and 10 mM NaH2PO4 (pH 7.0).
Samples were dissolved to a duplex concentration of 0.5 mM in 500 μL of buffer containing 10 mM NaH2PO4, 100 mM NaCl, and 5 μM Na2EDTA (pH 5.2; uncorrected for deuterium isotope effects).
Under normal conditions, both the wild-type sequence and the SNP-containing sequence will hybridize with the capture oligo, but with different reaction kinetics and equilibrium duplex concentrations.
These slower migrating species form at the high duplex concentrations used in the UV-shadowing gel experiments (Figure 2, left) as well as in SYBR Green-stained gels loaded with lower DNA concentration samples (Figure 2, right).
RNA duplex concentrations were determined by measuring absorbance at 260 nm and calculating concentrations using extinction coefficients provided by the manufacturer.
Synthesised siRNA duplex concentrations were measured by NanoDrop ND-1000 spectrophotometer (BCM, Houston, TX, USA) then diluted as needed.
Experiments were generally conducted at a hairpin duplex oligomer concentration of 3 × 10 6 M. For experiments with complexes, a ratio of two compounds per oligomer duplex was generally used.
