Sentence examples for dump channel from inspiring English sources

Briefly, aliquots of whole blood were labeled with a cocktail of monoclonal antibodies (mAbs) including anti-CD14-phycoerythrine (PE -Cy5 and anti-CD19-PE -Cy5for andump chanti-CD19-PE-Cy5-PE as a marker for type-1 myeloid DC (MDC1), CD141/BDCa-3-APC (for Mdump and channelDCanti-CD1c-PE plasmacytoid DC (PDC) [7].

For multi-parameter flow assays, a "dump channel" of cells positive for CD14 and CD19 (APC-Cy7) was used to improve specificity, and live/dead blue reagent (Invitrogen) was used to exclude dead cells.

Other reagents included biotinylated Abs against dump channel markers: Thy1 (Abcam), F4/80 (Abcam), CD11c, Gr-1, TER-119, and NK-1.1, secondary staining SA-Texas Red conjugate; v-amine live/dead violet dye (Molecular Probes), and Fc block anti-CD16/32 anti-CD16/32

Cells were then simultaneously stained with anti-CD3 mAb PE-Cy7 (BD Bioscience), anti-CD8 mAb PerCP-Cy5.5 (BD Bioscience), anti-CD4 mAb, anti-CD14 and anti-CD19 (all V450 conjugated; "dump channel"; BD Pharmingen), incubated in the dark for 30 minutes at room temperature (RT), and then washed.

FITC was used as a dump channel to exclude all CD4+ and CD19+ cells from the analysis.

We also established a dump channel (CD3, CD16, CD19, CD33) to exclude cells not of interest for the analysis.

However, because these non-specific events often fall in regions of interest, to find and exclude this cell subset manually generally requires a significant amount of time spent back-gating, looking where events in a region fall on other projections, or inclusion of more markers in the panel, such as viability dyes or dump channels.

Following enrichment, cells were stained with anti-mouse CD4-PE-Cy7, CD25-PE-Cy5, CD62L-FITC, and CD8-APC-Cy7 (as a dump fluorescence channel).

In a way they could just dump the channels and put up a list of embed codes.

If you have a purist small-company fund that dumps Clear Channel and a separate midsize fund that picks it up, you will end the day with a potential tax liability.

An underground drainage channel dumps wastewater through a pipe into the canal behind the school.