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The pH was kept constant in bioreactor fermentations due to titration with NaOH and H3PO4.
Thus, under conditions of elevated levels of WIT in Smn heterozygotes, it is possible that normal BMP signaling at the NMJ is altered, perhaps due to titration of the BMP ligand, thereby resulting in NMJ defects.
Thus, we hypothesize that the change observed in the pH-titrations monitored by the ANS-fluorescence of PsTRXh1, is due to titration of His23, which motivates local rearrangement and solvent-exposure of residues.
There was an acidic titration (which was also monitored by ANS-fluorescence and SEC, see below) that should be due to titration of an Asp and/or Glu residues [57]; however, due to the absence of an acidic baseline, we could not determine reliably its pKa (Fig. 3A).
The inhibition of ADCC was not due to titration or degradation of the antibody.
Another possible explanation could be that the cellulase genes were down-regulated due to titration of their activating transcription factors [ 37].
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Stabilization of HIF-1α by p160 may be due to the titration of VHL or HPHs.
This might be due to the titration of the replication factors by the firing of many additional sites.
According to the authors, the apparent superiority of Daytrana® observed during the study, however, is more likely to be due to careful titration and clinical monitoring for the study duration rather than to the product itself [ 46, 47].
These results seemed specific and were not due to GAL4 titration as numerous transgenes inserted at the same locus revealed no effect or opposite effects to PERK and ATF4 depleting transgenes.
Therefore, the altered pattern of MBNL1 expression may further contribute to the multisystemic manifestation of DM not only through the mere loss-of-function due to the titration of the RNA-binding proteins by the CUG expansions.
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