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With appropriate criteria for avoiding false positives due to sequencing errors, next-generation technologies can provide novel insights into genome-wide aspects of mtDNA heteroplasmy.
Additional polymorphisms due to sequencing errors could be identified by inspecting raw sequence data.
To reduce erroneous sample assignment due to sequencing error, all barcodes differ by at least two nucleotides.
Therefore the frequencies of mismatches due to sequencing errors are expected to be comparable across ESTs for the same genes.
However, increasing the sequence depth also directly affects the false discovery rate due to sequencing errors, further emphasizing the need to use stringent sequence quality parameters.
This study showed, however, that sequence differences due to sequencing errors of ESTs are at a level similar to that of allelic variation or even lower.
The first model assumed that the entire population had only one base at this position and that all disagreements were therefore due to sequencing errors.
This was partly due to sequencing errors.
These discrepancies could be due to sequencing errors.
Thus, most discrepancies with their source genomes are due to sequencing errors and low coverage.
Its purpose was to remove variants likely due to sequencing errors.
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