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If all putative peptides were to be detected in an MS experiment, there could be an overlap of 9,328 peptides due to sequence similarity between the two proteomes.
Locked nucleic acids (LNA) based real time PCR was used in particular situations where there are difficulties in primer design due to sequence complexity.
CONCLUSIONS Our analyses suggest that, beyond variation due to sequence counting, technical variation from sample processing can obscure temporal variation from biological sources in artificial gut studies.
We did not determine however, whether this was due to sequence mosaicism or horizontal gene transfer.
Neither ε nor α genes could be found, most likely due to sequence gaps.
Genotype 5 showed the weakest band, probably due to sequence changes in the antibody recognition sites.
Most likely, this deviation is due to sequence bias in the library.
We used an electronic mask to eliminate probes that behaved poorly due to sequence divergence.
In contrast, presumably due to sequence divergence, HPV45 was not cross-neutralized by any of the anti-HPV16 L2 antibodies.
The switch regions of γ2, γ3, and γ9 could not be identified, most likely due to sequence gaps.
However, we cannot exclude that phenotypic differences are not due to sequence variations in the PilB proteins.
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